source link PG NEET Microbiology MCQs 11 – Sterilisation and Disinfection

follow url 1. Sterilization accuracy is assessed with

  1. Clostridium perfringenes
  2. Geobacillus stereothermophilus
  3. Staphylococcus aureus
  4. Bacillus subtilis subsp Niger

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enter 2. Best method of sterilization by steam under pressure

  1. hor air oven
  2. boiling
  3. autoclaving
  4. tyndalisation

go to site Ans (3)

Tramadol Online Consultation Uk 3. Who is the person in the picture he was called “the father of modern and antiseptic surgery”

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  1. Robert Koch
  2. Joseph Lister
  3. Loius Pasteur
  4. Hippocrates

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http://geomore.com/sedimentary-rocks/ 4. Spore of which bacteria are used as sterilization control of  plasma sterilization

  1. B subtilis
  2. B pumilis
  3. Cl tetani
  4. B stereothermophilus

follow Ans (4)

5. The electron microscope was found by

  1. Robert Koch
  2. Joseph Lister
  3. Leeuwenhoek
  4. Ernst Ruska

Ans (4)

6. CLED medium is preferred over MacConkeys agar in relation to the culture of organism involved in urine infection. The reason is

  1. it prevents swarming of Proteus
  2. differentiates LF and NLF
  3. promotes staphylococcus streptococcus and candida
  4. identifies Pseudomonas

Ans (3)

7. Which of the following is most resistant to sterilization

  1. cysts
  2. prions
  3. spores
  4. viruses

Ans (2)

8. ——- ribosome is present in prokaryotes and ——– ribosome is present in eukaryotes

  1. 70s/80s
  2. 80s/70s
  3. 60s/70s
  4. 70s/60s

Ans (1)

9. Which of the following culture medium combination is/are true

  1. Thayer Martin media – Gonorrhoea
  2. chocolate agar – enriched media
  3. LJ medium – Mycobacterium tuberculosis
  4. Mueller Hinton agar – Corneybacterium diphtheriae
  5. MacConkey’s agar – nonlactose fermenters from colorless colonies

Ans (4)

10. Low-level disinfectant is

  1. benzalkonium chloride
  2. isopropyl alcohol
  3. glutaraldehyde
  4. hydrogen peroxide

Ans (1)

11. The method by which you cant see cell wall is

  1. reaction with specific antibodies
  2. electron microscopy
  3. differential staining
  4. microdissection
  5. simple microscopy

Ans (5)

12. TSI is

  1. selective
  2. enrichment
  3. enriched
  4. composite

Ans (4)

13. According to Spaulding classification arthroscope and laparoscopic instruments are under

  1. critical term
  2. semi-critical term
  3. noncritical term

Ans (1)

14. The wrong statement about bacterial cell wall is

  1. gram-negative bacteria have a outer membrane peptidoglycan and lipoprotein
  2. teichoic acid is present in gram-positive bacteria
  3. LPS is responsible for endotoxic activity and O antigen specificity in gram-positive bacteria
  4. peptidoglycan layer is thicker in gram-positive than gram-negative bacteria

Ans (3)

15. Selective media for shigella

  1. wilson Blair
  2. TCBS
  3. DCA
  4. blood agar

Ans (3)

16. False in Spaulding classification

  1. non-critical items also included in the classification
  2. semi-critical items – contact with the mucous membrane
  3. semi-critical items  – needs low disinfectant
  4. cardiac catheter eg: of a critical item

Ans (3)

17. In which phase does the cell divide exponentially in a bacterial growth curve

  1. lag phase
  2. log phase
  3. stationary phase
  4. decline phase

Ans (2)

18. Recommended transport medium for stool specimen suspected to contain enteric pathogens is

  1. Amie’s medium
  2. buffered glycerol saline medium
  3. MacConkey’s medium
  4. blood agar

Ans (2)

19. The surgical blade is sterilized by

  1. autoclave
  2. gamma radiation
  3. hot air oven
  4. steaming

Ans (1)

19. Bacillus stearothermophilus is an

  1. mesophilic
  2. thermophilic
  3. psychrophilic
  4. none of the others

Ans (2)

20. Robertson cooked meat medium is an example of

  1. enriched medium
  2. enrichment medium
  3. nutrient medium
  4. anaerobic medium

Ans (4)

21. Boiling of milk is an example of

  1. concurrent disinfection
  2. precurrent disinfection
  3. terminal disinfection
  4. sterilization

Ans (2)

22. Tyndalisation is done by

  1. exposure at 100° for 20 minutes for 3 consecutive days
  2. exposure at 100° for 10 minutes for 3 consecutive days
  3. exposure at 100° for 20 minutes for 5 consecutive days
  4. exposure at 120° for 20 minutes for 3 consecutive days

Ans (1)

23. Smith Nagouchi’s media is used for

  1. salmonella
  2. klebsiella
  3. spirochaetes
  4. bacillus

Ans (3)

24. All the following are used to check the efficiency of disinfectant except

  1. Chick Martin test
  2. Riedel Walker test
  3. Hugh Leifson test
  4. Kelsey Sikes test

Ans (3)

25. Phenol and alcohol acts by

  1. disrupting the cell membrane
  2. by modifying the protein
  3. both
  4. none

Ans (1)

26. Human anatomical waste is disposed of in ———— cover

  1. yellow
  2. blue
  3. red
  4. black

Ans (1)

27. To prevent swarming the % of agar in nutrient agar has to be increased by

  1. 2%
  2. 4%
  3. 6%
  4. 8%

Ans (3)

28. Kovac’s reagent used in indole test contains

  1. para-dimethyl amino benzaldehyde
  2. amyl or isoamyl alcohol
  3. concentrated HCl
  4. all the others

Ans (4)

29. Sharp metals are discarded into which of the following colors in biomedical waste disposal

  1. blue
  2. red
  3. yellow
  4. white

Ans (4)

30. PCR is used for

  1. detect target plasmids
  2. amplify a small amount of DNA
  3. seal the cut ends of DNA
  4. cleave the bacterial plasmids

Ans (2)

31. Transformation is

  1. direct transfer fo free DNA
  2. transfer of a portion of DNA from one bacterium to anther by bacteriophage
  3. the male donor makes a physical contact with a female for transfer
  4. none of the others are right

Ans (1)

32. which of the following is a subunit vaccine

  1. hepatitis B
  2. HPV vaccine
  3. both are right
  4. none are right

Ans (3)

33. Enzyme used in PCR are

  1. restriction endonucleases
  2. DNA polymerases
  3. alkaline phosphate
  4. RNA polymerases
  5. reverse transcriptase

Ans (2)

34. False about transposon

  1. they are called jumping genes
  2. discovered by Barbara McClintock
  3. small transposons are called insertion sequence
  4. none are false

Ans (4)

35. Test which cannot be sued to calculate MIC is

  1. tube dilution
  2. agar dilution
  3. Kirby Bauer test
  4. all

Ans (3)

36. All the following are required in PCR except

  1. deoxyribonucleotides
  2. thermostable enzyme/DNA polymerase
  3. dideoxyribonucleotides
  4. magnesium/ssDNA/template DNA

Ans (3)

37. PCR is used to ———— DNA

  1. diminish
  2. vanish
  3. modify
  4. amplify

Ans (4)

38. E test is

  1. also known as epsilometer test
  2. done by diffusion and dilution method
  3. it is a quantitative method to find out antimicrobial susceptibility
  4. it finds the minimum inhibitory concentration
  5. All are right

Ans (5)

39. Chromosomal mutation can be identified by all except

  1. single strand polymorphism
  2. agarose gel electrophoresis
  3. denaturing gradient gel electrophoresis
  4. dideoxynucleotide trail sequencing

Ans (2)

40. RNA dependent DNA  polymerase is

  1. reverse transcriptase
  2. RNA polymerase
  3. integrase
  4. proviral DNA

Ans (1)

41. Bacteriological examination of milk is done by the following test

  1. methylene blue reduction test
  2. phosphatase test
  3. resazurin test
  4. all the others are right

Ans (4)

42. For PCR thermus auqzicus plant is used to prepare

  1. DNA polymerase
  2. RNA polymerase
  3. primers
  4. restriction of endonucleases II

Ans (1)

43. Northern blotting is used for detection of

  1. RNA
  2. DNA
  3. protein
  4. fat

Ans (1)

44. Presence of enterococcus in water is a sign of

  1. old fecal contamination
  2. old blood contamination
  3. recent fecal contamination
  4. recent bloodcontaminationn

Ans (3)

45. DNA hybridization is called

  1. southern blot
  2. northern blot
  3. eastern blot
  4. western blot

Ans (1)

46. Conjugation caused by plasmid was first found in which organism

  1. E coli
  2. pseudomonas
  3. rickettsiae
  4. V cholera

Ans (1)

47. The presumptive coliform count should be this level to call a water satisfactory

  1. 0/100ml
  2. 1-3/100ml
  3. 4-6/100ml
  4. 7-10/100ml

Ans (2)

48. Which of the following structures is required in the microscope for taking this type of image

Image result for fluorescence microscopy images

  1. dark field condenser
  2. phase plate
  3. dichroic mirror
  4. cathode ray tube

Ans (3)

49. Constituents of MacConkeys agar is

  1. peptone water
  2. lactose
  3. sodium tetracholate
  4. agar
  5. all the others are right

Ans (5)

50. The differential coliform test was found by

  1. Christian Eijkman
  2. Robert Pasteur
  3. Edward Jenner
  4. Hercules

Ans (1)

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